Activity of ozone against leishmania major in vitro condition

Document Type : Original Article


1 Department of Laboratory sciences, Faculty of Paramedical, Kashan University of Medical Sciences, Kashan, Iran

2 Department of Parasitology, Faculty of Medical Sciences, Kashan University of Medical Sciences, Kashan, Iran AND Infectious Diseases Research Center, Kashan University of Medical Sciences, Kashan, Iran



Objective: Cutaneous leishmaniasis is one of the most common diseases in the world and various studies in the field of its treatment are of special importance. This study was aimed to investigate the effects of ozone on Leishmania major.
Methods: Leishmania major promastigotes were exposed to ozone flow at four different concentrations (5, 10, 20, 30 and 40 ppm) at five times in 5, 10, 20, 30 and 40 minutes. The DNA of the treated promastigotes that were affected by ozone was extracted and the DNA fragmentation was evaluated. Induction of apoptosis in the treated promastigotes of L.major was analyzed by a specific kit. Scanning electron microscopy was also used to analyze ultrastructural changes of treated promastigotes.
Results: The highest fatality (100%) was obtained 30 minutes after exposure to 40 ppm ozone gas, and the lowest fatality was 16.14% after exposure to 5 ppm ozone gas flow after 5 minutes. The DNA of the treated parasites was degraded and the morphology of the promastigotes was significantly changed in the body and flagella. Induction of apoptosis was also observed in promastigotes.
Conclusion: Ozone has anti-Leishmania activity and induces apoptosis in Leishmania major, ultrastructural changes in Leishmania major are also clearly observed after exposure to ozone. Overall, ozone can be investigated as an anti-leishmania agent in further studies.


Main Subjects

Articles in Press, Accepted Manuscript
Available Online from 03 April 2024
  • Receive Date: 21 September 2023
  • Revise Date: 03 December 2023
  • Accept Date: 01 April 2024