Role of Class I, II and III Integrons in Multidrug Resistance in Pseudomonas aeruginosa Isolated from Nosocomial Hospital

Document Type : Original Article


1 Department of Pharmacodynamy & Toxicology, Faculty of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran

2 Department of Microbiology, Faculty of Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran


Aims The increasing usage of antibiotics can cause resistance to the treatment of infections,
which can caused by bacteria, e.g. Pseudomonas aeruginosa.The aim of this study was to
trace the class I, II and III integrons in isolates of P. aeruginosa of nosocomial infection and
determining the antibiotic resistance pattern of the bacteria.
Instrument & Methods In this cross-sectional study, 100 Pseudomonas aeruginosa clinical
isolates of infected wounds, bedsores, burns, urinary tract infections and respiratory tract
infections were collected from patients of 3 Isfahan City hospitals, Iran (Al Zahra, Kashani,
Shariati) in 2015. After identification tests and antibiogram, integrons class I, II and III were
detected by M-PCR method.Data analysis were performed in SPSS 16 software using Chisquare
and Fisher exact tests and the relationship between the presence of class III, II, I was
calculated by M-PCR test.
Findings All isolates had multiple antibiotic resistances. The highest antibiotic resistance
was to Tetracycline (85%) and the lowest to Norfloxacin (12.5%). There were significant
differences between class I and the two other classes of integrons (p=0.036). There was a
statistically significant difference between the presence of blaTEM gene in Pseudomonas
aeruginosa with other coding genes for antibiotic resistance (p=0.029).
Conclusion Pseudomonas aeruginosa isolates are multi-drug resistant and almost all isolates
from clinical infections have class I, II and III Integrons.