Diagnosis of Clinical and Laboratory Findings of Brucellosis in Isfahan

Document Type : Original Article


1 Department of Pharmacodynamics' and Toxicology, Faculty of Pharmacy, Mashhad University of Medical Sciences, Mashhad,

2 Nourdanesh Institute of Higher Education of Meymeh, Isfahan,

3 Department of Microbiology, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran



Aims: Brucellosis is one of the important infectious factors in most areas of Iran and other developing countries. Brucellosis has different
clinical manifestations and should be considered in the differential diagnosis of infectious and noninfectious diseases. The aims of this
study was to determine the epidemiological, clinical findings of the brucellosis in patients based on blood culture and serological tests.
Materials and Methods: In this cross‑sectional study, 190 brucellosis patients admitted in Isfahan Hospital in 2016–2017 that were studied
based on positive immunological tests such as Wright, 2‑mercaptoethanol (2ME), and enzyme‑linked immunosorbent assay. Samples of blood
were cultured (BACTEC) and incubated at 37°C for 5 days and then on Brucella agar. In addition, clinical and laboratory characteristics
of brucellosis were done. The patients who had brucellosis (Coombs test ≥1/80 and 2ME ≥1/40) were selected. Data were analyzed using
SPSS statistical package. Results: About 62.5% of patients were female with mean age of 37.5% years. 54.8% of cases were living in urban
and 45.2% in rural areas. The most contagious seasons were spring. The most common transmission way was consuming of contaminated
dairy products (59.3%); however, some of the patients had a history of animal contacts. The most common symptoms were fever (65.2%),
arthralgia (68.1%), sweating (32%), malaise and fatigue (37.2%). The most common clinical signs were fever and peripheral arthritis. High
leukocytosis and elevated erythrocyte sedimentation rate (>20 mm/h) were reported in all of the cases. Elevated C‑reactive protein was detected
in 72.1% of patients. Conclusion: Brucellosis should be considered in the differential diagnosis of patients with prolonged fever, spondylitis,
or peripheral arthritis in endemic areas.